Reactome is a free, open-source, curated, and peer-reviewed biological pathway database. It provides comprehensive pathway data covering human biology and orthologous reactions for over 14 non-human species including mouse, rat, yeast, and fruit fly. The platform exposes two primary REST APIs — the Content Service for querying pathway data, molecular interactions, species comparisons, and reaction network visualization, and the Analysis Service for pathway enrichment analysis of gene and protein identifier lists. Both APIs are freely accessible without authentication, fully documented with OpenAPI (Swagger), and cross-referenced with major biological databases including NCBI, Ensembl, UniProt, KEGG, ChEBI, PubMed, and Gene Ontology. Data is authored by expert biologist researchers and maintained by Reactome editorial staff.
The Reactome Content Service provides programmatic access to all knowledgebase content including pathways, reactions, physical entities, complexes, and molecular interactions. I...
The Reactome Analysis Service enables pathway enrichment analysis for lists of gene or protein identifiers. Users can submit identifier lists via direct POST, file upload, or UR...
aid: reactome
name: Reactome
description: Reactome is a free, open-source, curated, and peer-reviewed biological pathway database. It provides comprehensive
pathway data covering human biology and orthologous reactions for over 14 non-human species including mouse, rat, yeast,
and fruit fly. The platform exposes two primary REST APIs — the Content Service for querying pathway data, molecular interactions,
species comparisons, and reaction network visualization, and the Analysis Service for pathway enrichment analysis of gene
and protein identifier lists. Both APIs are freely accessible without authentication, fully documented with OpenAPI (Swagger),
and cross-referenced with major biological databases including NCBI, Ensembl, UniProt, KEGG, ChEBI, PubMed, and Gene Ontology.
Data is authored by expert biologist researchers and maintained by Reactome editorial staff.
type: Index
position: Provider
access: Public
image: https://kinlane-images.s3.amazonaws.com/shared/apis-json/apis-json-logo.jpg
tags:
- Biological Pathways
- Bioinformatics
- Life Sciences
- Pathway Analysis
- Gene Enrichment
- Molecular Interactions
- Systems Biology
- Open Science
url: https://raw.githubusercontent.com/api-evangelist/reactome/refs/heads/main/apis.yml
created: '2026-06-13'
modified: '2026-06-13'
specificationVersion: '0.19'
apis:
- aid: reactome:content-service
name: Reactome Content Service API
description: The Reactome Content Service provides programmatic access to all knowledgebase content including pathways,
reactions, physical entities, complexes, and molecular interactions. It supports querying by identifier, retrieving pathway
hierarchies, exporting pathway diagrams in multiple formats (PNG, JPG, SVG, PDF, SBML, SBGN), searching via Solr full-text
search, querying PSICQUIC interactor databases, and mapping external identifiers to Reactome entities. The API covers
human biology as well as orthologous reactions for over 14 species. No authentication is required and the API is free
to use for research purposes.
humanURL: https://reactome.org/dev/content-service
baseURL: https://reactome.org/ContentService
tags:
- Pathways
- Reactions
- Molecular Interactions
- Diagram Export
- Species Orthology
- Search
- Interactors
properties:
- type: Documentation
url: https://reactome.org/dev/content-service
- type: OpenAPI
url: https://reactome.org/ContentService/v3/api-docs
- type: OpenAPI
url: https://raw.githubusercontent.com/api-evangelist/reactome/refs/heads/main/openapi/content-service.json
- type: SwaggerUI
url: https://reactome.org/ContentService/
- aid: reactome:analysis-service
name: Reactome Analysis Service API
description: The Reactome Analysis Service enables pathway enrichment analysis for lists of gene or protein identifiers.
Users can submit identifier lists via direct POST, file upload, or URL reference. The service automatically detects overrepresentation
analysis for identifier lists or expression analysis for tab-separated values with numeric expression data. Results include
pathway hit data, p-values, false discovery rate (FDR), and entity and reaction counts. Analysis results are assigned
a token valid for 7 days, allowing subsequent retrieval, filtering by species, pagination, and PDF report generation without
resubmitting data. Species comparison against Homo sapiens is also supported.
humanURL: https://reactome.org/dev/analysis
baseURL: https://reactome.org/AnalysisService
tags:
- Pathway Enrichment
- Overrepresentation Analysis
- Expression Analysis
- Species Comparison
- Gene Lists
- Protein Identifiers
properties:
- type: Documentation
url: https://reactome.org/dev/analysis
- type: OpenAPI
url: https://reactome.org/AnalysisService/v3/api-docs
- type: OpenAPI
url: https://raw.githubusercontent.com/api-evangelist/reactome/refs/heads/main/openapi/analysis-service.json
- type: SwaggerUI
url: https://reactome.org/AnalysisService/
common:
- type: Website
url: https://reactome.org/
- type: Documentation
url: https://reactome.org/documentation
- type: DeveloperPortal
url: https://reactome.org/dev
- type: DataModel
url: https://reactome.org/documentation/data-model
- type: FAQ
url: https://reactome.org/documentation/faq
- type: GitHub
url: https://github.com/reactome
- type: Blog
url: https://reactome.org/about/news
- type: License
url: https://reactome.org/license
- type: Contact
url: https://reactome.org/about/contact-us
- type: About
url: https://reactome.org/about
maintainers:
- FN: Kin Lane
email: kin@apievangelist.com
