# Opentrons Python Protocol API

**Canonical:** https://apis.io/apis/opentrons/opentrons-python-protocol-api/  
**Provider:** Opentrons — https://apis.io/providers/opentrons/  
**Base URL:** https://docs.opentrons.com  
**Documentation:** https://docs.opentrons.com/v2/

Opentrons Python Protocol API is one of 10 APIs that [Opentrons](https://apis.io/providers/opentrons/) publishes on the [APIs.io](https://apis.io/) network. Tagged areas include Python, Protocol, Liquid Handling, Lab Automation, and Hardware Modules. The published artifact set on APIs.io includes API documentation, a getting-started guide, an API reference, and a GitHub repository.

The Opentrons Python Protocol API is an open-source Python framework for writing automated biology lab protocols that run on Opentrons Flex and OT-2 robots. It provides programmatic control over pipettes, labware, deck configuration, hardware modules (thermocyclers, heater-shakers, temperature modules, magnetic modules, absorbance readers), and supports dynamic decision-making and custom integrations. Protocols are executed by the robot server and can be controlled via the HTTP API or Opentrons App.

## Machine-readable artifacts (7)

- **Documentation** — https://docs.opentrons.com/v2/
- **GettingStarted** — https://docs.opentrons.com/v2/new_quickstart.html
- **APIReference** — https://docs.opentrons.com/v2/new_protocol_api.html
- **ProtocolExamples** — https://docs.opentrons.com/v2/new_examples.html
- **GitHubRepository** — https://github.com/Opentrons/opentrons
- **PyPI** — https://pypi.org/project/opentrons/
- **APIsJSON** — https://raw.githubusercontent.com/api-evangelist/opentrons/refs/heads/main/apis.yml

## Other Opentrons APIs (9)

- [Opentrons Attached Instruments API](https://apis.io/apis/opentrons/opentrons-attached-instruments-api/)
- [Opentrons Attached Modules API](https://apis.io/apis/opentrons/opentrons-attached-modules-api/)
- [Opentrons Flex Deck Configuration API](https://apis.io/apis/opentrons/opentrons-flex-deck-configuration-api/)
- [Opentrons Health API](https://apis.io/apis/opentrons/opentrons-health-api/)
- [Opentrons Protocol Management API](https://apis.io/apis/opentrons/opentrons-protocol-management-api/)
- [Opentrons Robot API](https://apis.io/apis/opentrons/opentrons-robot-api/)
- [Opentrons Run Management API](https://apis.io/apis/opentrons/opentrons-run-management-api/)
- [Opentrons Simple Commands API](https://apis.io/apis/opentrons/opentrons-simple-commands-api/)
- [Opentrons System Control API](https://apis.io/apis/opentrons/opentrons-system-control-api/)

## Tags

Python, Protocol, Liquid Handling, Lab Automation, Hardware Modules, Open-Source

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Profiled by [API Evangelist](https://apievangelist.com) and published on [APIs.io](https://apis.io/apis/opentrons/opentrons-python-protocol-api/). The API's provider profile, Kin Score and agent-readiness rating are at https://apis.io/providers/opentrons/.