# Benchling Nucleotide Alignment API

**Canonical:** https://apis.io/apis/benchling/benchling-nucleotidealignment-api/  
**Provider:** Benchling — https://apis.io/providers/benchling/  
**Base URL:** https://{tenant}.benchling.com/api/v2  
**Documentation:** https://docs.benchling.com/docs/developer-platform-overview

Benchling Nucleotide Alignment API is one of 203 APIs that [Benchling](https://apis.io/providers/benchling/) publishes on the [APIs.io](https://apis.io/) network, described by a machine-readable OpenAPI specification. Tagged areas include NucleotideAlignment. The published artifact set on APIs.io includes an OpenAPI specification, API documentation, an API reference, authentication docs, rate-limit docs, and a changelog.

Represents a multiple sequence alignment of nucleotide sequences (DNA or RNA). Nucleotide alignments are used to compare sequence reads against a reference template for verification (see AlignmentType TEMPLATE) or to align multiple sequences for consensus building (see AlignmentType CONSENSUS). Each alignment contains a referenceSequence (the template or consensus) and a collection of AlignedNucleotideSequence entries representing the individual sequences being compared. Alignments are commonly used in Sanger sequencing verification workflows to confirm that a cloned construct matches the expected design.

## Operations (9)

| Method | Path | Summary |
|---|---|---|
| GET | `/nucleotide-alignment/items` | List NucleotideAlignment items |
| DELETE | `/nucleotide-alignment/{nucleotide_alignment_id}` | Delete NucleotideAlignment |
| GET | `/nucleotide-alignment/{nucleotide_alignment_id}` | Get NucleotideAlignment by ID |
| GET | `/nucleotide-alignment/{nucleotide_alignment_id}/aligned-nucleotide-sequences/items` | List AlignedNucleotideSequence items |
| POST | `/nucleotide-alignment:batch-delete` | Batch delete NucleotideAlignment |
| POST | `/nucleotide-alignment:create-consensus-alignment` | Create a consensus nucleotide alignment |
| POST | `/nucleotide-alignment:create-template-alignment` | Create a template nucleotide alignment |
| GET | `/tasks/nucleotide-alignment/create-consensus-alignment/{task_id}` | Poll async task status |
| GET | `/tasks/nucleotide-alignment/create-template-alignment/{task_id}` | Poll async task status |

## Machine-readable artifacts (15)

- **OpenAPI** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/openapi/benchling-nucleotidealignment-api-openapi.yml
- **OpenAPIRemote** — https://benchling.com/api/v3/openapi.yaml
- **Documentation** — https://docs.benchling.com/docs/developer-platform-overview
- **APIReference** — https://benchling.com/api/v3/reference
- **Authentication** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/authentication/benchling-authentication.yml
- **OAuthScopes** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/scopes/benchling-scopes.yml
- **Conventions** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/conventions/benchling-conventions.yml
- **ErrorCatalog** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/errors/benchling-problem-types.yml
- **DataModel** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/data-model/benchling-data-model.yml
- **Webhooks** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/asyncapi/benchling-webhooks.yml
- **RateLimits** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/rate-limits/benchling-rate-limits.yml
- **Lifecycle** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/lifecycle/benchling-lifecycle.yml
- **ChangeLog** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/changelog/benchling-changelog.yml
- **StabilityPolicy** — https://docs.benchling.com/docs/stability-guidelines
- **SDKs** — https://raw.githubusercontent.com/api-evangelist/benchling/refs/heads/main/packages/benchling-packages.yml

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- [Benchling AA Sequences API](https://apis.io/apis/benchling/benchling-aa-sequences-api/)
- [Benchling Apps API](https://apis.io/apis/benchling/benchling-apps-api/)
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- [Benchling Assay Runs API](https://apis.io/apis/benchling/benchling-assay-runs-api/)
- [Benchling Audit API](https://apis.io/apis/benchling/benchling-audit-api/)
- [Benchling Authentication API](https://apis.io/apis/benchling/benchling-authentication-api/)
- [Benchling Blobs API](https://apis.io/apis/benchling/benchling-blobs-api/)
- [Benchling Boxes API](https://apis.io/apis/benchling/benchling-boxes-api/)
- [Benchling Codon Usage Tables API](https://apis.io/apis/benchling/benchling-codon-usage-tables-api/)
- [Benchling Connect API](https://apis.io/apis/benchling/benchling-connect-api/)
- [Benchling Containers API](https://apis.io/apis/benchling/benchling-containers-api/)
- [Benchling Custom Entities API](https://apis.io/apis/benchling/benchling-custom-entities-api/)

## Tags

NucleotideAlignment

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Profiled by [API Evangelist](https://apievangelist.com) and published on [APIs.io](https://apis.io/apis/benchling/benchling-nucleotidealignment-api/). The API's provider profile, Kin Score and agent-readiness rating are at https://apis.io/providers/benchling/.